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Boehringer Mannheim
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Jackson Laboratory
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Elabscience Biotechnology
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Yeasen Biotechnology
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Quantum Dot Inc
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Image Search Results
Journal: Biomolecules
Article Title: Adenovirus Fibers as Ultra-Stable Vehicles for Intracellular Nanoparticle and Protein Delivery
doi: 10.3390/biom12020308
Figure Lengend Snippet: The LHB protein is attached to AlexaFluor ® 594 streptavidin Nanogold ® by the means of the biotin-streptavidin strong non-covalent bond. Cell internalization studies of the LHB protein-streptavidin gold NP conjugate in HeLa ( A ) and NIH3T3 ( B ) cells are shown. The protein construct is labeled with NHS-fluorescein (green) and further to the streptavidin-gold NP which contains an exposed Alexa595 dye (red). Cell nuclei are stained with DAPI (blue). Z-stack measurements of the cells were also obtained to ensure that the protein conjugates localize inside the cells at the same level. ( 1 ) Only the nuclei (blue) and the AlexaFluor594 (red) filters are applied in the confocal microscope. ( 2 ) Only the nuclei (blue) and the fluorescein (green) filters are active. ( 3 ) All filters are applied and the green and red signals are co-localized mergings into a yellow–orange color, indicating the effective transport of the intact complex. Scale bar: 50 μm.
Article Snippet: LHB protein molecules labeled with NHS-fluorescein (green fluorescence) were incubated with the
Techniques: Construct, Labeling, Staining, Microscopy